Flavonol Sulfotransferase-Like cDNA Clone from Flaveria bidentis

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Partial Purification and Some Properties of Flavonol 7-Sulfotransferase from Flaveria bidentis.

A novel flavonol-specific sulfotransferase was partially purified from the shoot tips of Flaveria bidentis var. Angustifolia O.K. (Asteraceae) by chromatography on 3'-phosphoadenosine 5'-phosphate-agarose affinity column and chromatofocusing on Mono P. The latter step resulted in the separation of two isoforms, both of which exhibited expressed specificity for position 7 of quercetin 3,3'- and ...

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Identification and Biosynthesis of Glucosylated and Sulfated Flavonols in Flaveria bidentis

1. The flavonoid constituents o f Flaveria bidentis have been identified as the 3-O-glucosides of kaempferol, 6 -m ethoxykaem pferol and 6 -methoxyquercetin (patuletin), as well as six flavonol sulfate esters belonging to quercetin and isorhamnetin. The two latter glucosides are reported here for the first time in this species; whereas quercetin-3,7-disulfate, is a novel compound from nature. 2...

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Partial Purification and Characterization of Three Flavonol-Specific Sulfotransferases from Flaveria chloraefolia.

Three distinct flavonol-specific sulfotransferases were partially purified from the shoot tips of Flaveria chloraefolia A. Gray by fractional precipitation with ammonium sulfate, followed by gel filtration on Sephacryl S-200, 3'-phosphoadenosine 5-phosphate-Agarose affinity chromatography and chromatofocusing on Mono P. These enzymes exhibited expressed specificity for positions 3 of various fl...

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The Gene for the P-Subunit of Glycine Decarboxylase from the C4 Species Flaveria trinervia: Analysis of Transcriptional Control in Transgenic Flaveria bidentis

Glycine decarboxylase (GDC) plays an important role in the photorespiratory metabolism of plants. GDC is composed of four subunits (P, H, L, and T) with the P-subunit (GLDP) serving as the actual decarboxylating unit. In C3 plants, GDC can be found in all photosynthetic cells, whereas in leaves of C3-C4 intermediate and C4 species its occurrence is restricted to bundle-sheath cells. The specifi...

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The gene for the P-subunit of glycine decarboxylase from the C4 species Flaveria trinervia: analysis of transcriptional control in transgenic Flaveria bidentis (C4) and Arabidopsis (C3).

Glycine decarboxylase (GDC) plays an important role in the photorespiratory metabolism of plants. GDC is composed of four subunits (P, H, L, and T) with the P-subunit (GLDP) serving as the actual decarboxylating unit. In C(3) plants, GDC can be found in all photosynthetic cells, whereas in leaves of C(3)-C(4) intermediate and C(4) species its occurrence is restricted to bundle-sheath cells. The...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1995

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.107.3.1019